Coding

Part:BBa_K4131001:Design

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-09)


AccBC gene (C. glutamicum), codon optimized for E. Coli expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1331
    Illegal XhoI site found at 80
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 1062
    Illegal NgoMIV site found at 1552
    Illegal AgeI site found at 426
    Illegal AgeI site found at 928
    Illegal AgeI site found at 1711
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon optimized for E.Coli expression, and the restriction enzyme sites of XbaI, SpeI, EcoRI, PstI, and NotI are eliminated.


Source

The sequence is extracted and modified from the region 1499728-1501502 in Corynebacterium glutamicum genome (CP025534.1)

References